Sun14November1246PM 12

Do methods of sterilisation include the following?

True / False

Cellulose filtration Incorrect answer selected

Dry heat at 134°C for three minutes Correct

Steam at 80°C and subatmospheric pressure Correct

Gamma irradiation Correct

Super oxidised water Incorrect answer selected

Explanation

Moist heat (for example, autoclave) is a method of sterilisation and the process kills all organisms and spores. Examples include:

122°C at 1 atm for 30 minutes

126°C at 1.5 atm for 10 minutes, or

134°C at 2 atm for three minutes

to sterilise non-heat sensitive surgical instruments.

Steam is used to increase the temperature so dry heat needs to be at a higher temperature for a longer time, for example, 160°C for one hour, to have a similar effect to moist heat.

Dry heat is used to sterilise glassware and powders.

Super-oxidized water is a broad spectrum disinfectant used for decontaminating heat sensitive medical equipment (endoscopes), which is effective on bacteria and fungi and can be used for the control of nosocomial infection. It will not guarantee sterilisation of equipment as some spores and prions may remain.

Steam at low pressure and 80°C will only disinfect instruments, and it does not destroy spores. The addition of formaldehyde will cause sterilisation, and is used to sterilise heat sensitive equipment such as cannulae.

Gamma irradiation is emitted from a cobalt-60 source or an electron accelerator, and is an expensive but effective means of sterilising plastics prostheses.

Cellulose filtration is used to sterilise heat sensitive fluids but it may not remove viral particles.

Answer Statistics

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Average score: 51.49%

Times answered: 235